Uncleavable DUB Substrates

DUB-resistant di-ubiquitin

DUB-resistant, K11-linked Di-ubiquitin (Ub2): contains a wild-type C-terminus on the proximal ubiquitin.


DUB-resistant, K48-linked Di-ubiquitin (Ub2):  includes a K48R mutation in the distal ubiquitin and a wild-type C-terminus on the proximal ubiquitin.


DUB-resistant, K63-linked Di-ubiquitin (Ub2): contains a wild-type C-terminus on the proximal ubiquitin.