Ubiquitin conjugated to 7-Amino-4-MethylCoumarin (AMC)

The conjugation of the Carboxyl extremity of mature Ubiquitin with AMC quenches the intrinsic fluorescence of AMC.
Upon incubation with a protease recognizing Ubiquitin, such as USP2 or UCHL3, AMC is released and the increase in fluorescence can be measured using Ex380 nm and Em460 nm wavelengths.



Ubiquitin-AMC reference

Ubiquitin Human AMC - Size: 50 µg