Quantification

Ubiquitin is everywhere... Get it all!

UbiQuant : The first quantitative Ubiquitin sandwich ELISA kit:

The UbiQuant™ ELISA is a sandwich assay in which total Ubiquitin is captured in the wells of a pre-coated microtiter plate using a proprietary Ubiquitin binding reagent. Unbound protein is removed by washing and
then an anti-Ubiquitin monoclonal antibody (VU-2) is added. After removing unbound VU-2 antibody, bound antibody is measured using an enzyme linked
anti-mouse antibody.

Beyond the ordinary…..

Benefits

  • Precision: quantitate total Ubiquitin (mono- and poly-)
  • Accuracy: Answers in µg/ml, not arbitrary units
  • High sensitivity: ~7 ng/ml
  • Accurate reproducibility:  Std. Dev. 0.104 and CV 9.5%

When to use:

UbiQuant™ ELISA is intended for the accurate determination of the concentration of total ubiquitin (poly-+ mono-) in biological samples including cell lysates, tissue homogenates and plasma.

Principle:

UbiQuant™ ELISA is a sandwich assay in which total Ubiquitin is captured in the wells of a pre-coated microtiter plate using a proprietary ubiquitin binding reagent